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To address these challenges, various delivery methods have been investigated, including viral vectors, non-viral vectors, and rational design strategies such as chemical modifications, cationic liposomes, polymers, nanocarriers, and biocoupled siRNAs, aimed at enhancing stability and intracellular delivery (Liu F

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In the cytosol, the GSH-to-GSSG ratio is high, consistent with a reducing environment, whereas in the endoplasmic reticulum (ER), the GSH redox couple is in a much more oxidized state, in line with the role of this organelle in protein disulfide bond formation (269)